In this project, biochemical, immunological and cell biological analyses will be carried out to accompany the production of an alternative full medium (AV). The AV is generated by the interaction of DMEM medium, the C3A secttom and deligandised albumin and is intended to replace the addition of FKS to the basic medium. The AV will be used for the cultivation of C3A cells and other human, industrially relevant cell lines.