This application aims to implement an advanced system for the production, purification and analysis of proteins both when isolated as well as in their cellular environment, which will allow BIFI researchers to make a qualitative leap in their research lines aimed at the discovery of drugs for various human diseases including different types of cancer, amyloid (Parkinson) and infectious diseases. A bottleneck in this field is the availability of advanced cellular models that allow conclusive studies while minimizing the use of animal models prior to embark on clinical trials. With this goal in mind, this application includes advanced equipment for eukaryotic cell culture, ranging from large-scale suspension models to complex organoids. This infrastructure will also facilitate both the production, the purification and the quality control of eukaryotic proteins, otherwise unstable or non-functional when expressed in bacteria, as well as the study of its function in vitro, in vivo and in silico. The requested infrastructure includes state-of-the-art incubators and electroporators for large-scale cultivation and transfection of eukaryotic cells, as well as modern systems for the generation of organoids and organs-on-chips, advanced equipment for protein purification, equipment for the determination of protein homogeneity and size, and computer graphic processing units for the integration of the results of the "wet lab" with modern computational analyses.